Proline Relationship with Protein in Seedlings Under Induced Stress in the Presence of Putrescine

Authors:

Pushpa C. Tomar (Manav Rachna International University, Faridabad)

Keywords: Indian mustard; Putrescine; Salinity; Proline.

Abstract:

Seedlings of Indian mustard (Brassica juncea, L.cv. RH-30) grown in controlled condition (irradiance 75 W m^-2, RH 60-70% and temp. 25 ± 2℃) for 7d and watered with Hoagland's solution containing different level of NaCl (70, 175 mmol/L NaCl) with or without putrescine (PUT, 1 mmol/L) were examined for PUT amelioration of NaCl induced inhibition in seedling growth. Proline and protein were also examined in leaves as well as in roots to find out their relationship in dark conditions. Salinity caused reduction in seedling growth and biomass accumulation was parallel to increased proltne accumulation in leaf and root tissues which were reversed significantly by PUT. This finding suggests that PUT might be activating antioxygenic enzymes and elevating antioxidants there by controlling free radical generation. hence preventing membrane peroxidation and denaturation of bio-molecules resulting into improved seedling growth under salinity.