in-vitro Multiplication of psoralea corylifolia an Endangered Medicinal Plant

Authors:

Gulshan K. Chhabra (Advanced Center for Biotechnology, M.D. University, Rohtak)

Deepika V. Aggarwal (Department of Botany, University of Delhi, New Delhi)

Pawan K. Jaiwal (Corresponding author, Advanced Center for Biotechnology, M.D. University, Rohtak)

Keywords: Psoralea corylifolia-micro-propagation from in vitro-raised shoots-nodal segments-6
benzyl amino purine-rooting.

Abstract:

A protocol for in-vitro mass multiplication of Psoralea corylifolia plants through direct organogenesis from various seedling explants is described. The cotyledonary node explants developed the maximum number of adventitious shoots (5.0‡0.5) per explant in 67% of cultures on MS containing 10MM 6-benzyl amino purine (BAP) within four weeks. Each shoot provided 2-3 culturable nodal segments. Shoots were further multiplied (5-6 shoots per explant) by culture of nodal segments. Shoots rooted on MS medium containing 5 AM indole-3 butyric acid with 100% efficiency. The plantlets showed 80% survival on transplantation in soil and were morphologically normal like the seed-raised plants.