Measurement of Serum Glucose with Oxidase Immobilized onto Onion Membrane

Authors:

Sanjay Kumar (M.D. University, Rohtak)

C.S. Pundir (M.D. University, Rohtak)

Keywords: Glucose, glucose oxidase, immobilization, onion membrane, serum.

Abstract:

A reusable strip of glucose oxidase (GOD) from Aspergillus was prepared by immobilizing the enzyme onto onion membrane affixed on plastic strip with a conjugation yield of 2.0 mg/cm and 63% retention of its initial activity. The immobilized enzyme showed maximum activity at pH 6.5, when incubated at 37°C for 15 min. A method for measurement of serm glucose was developed employing this strip. The minimum detection limit of the method was 5.0 mg/dl. Within batch and between batch CV for serum glucose were <5% and <6%. A good correlation(r=0.90) was found between serum glucose by commercial enzo kit method and the present method. No significant loss in the strip was observed after its 100 regular uses over a period of 40 days, when stored in reaction buffer at 4°C. This method has advantage. over commercial enzymic colourimetric method that it provides reuse of glucose oxidase with ease.